Cloning and characterization of developmentally differentially expressed novel genes from developing rat cerebral cortex / 대한해부학회지

The structural complexity and heterogeneity of cerebral cortex have been obvious since the earliest days of light microscopy. In fact, if there is one word that captures many of the key attributes of cortical structures, it is diversity. Neurodevelopmental approach is the one of the effective ways to understand complicated structures of cerebral cortex. In this experiment, as a first step to clone the genes related with development of cerebral cortex, the developmentally differentially expressed genes were cloned from developing rat brain with ordered differential display PCR(ODD-PCR). Novel genes were screened from these cloned genes by sequencing and sequence analysis with blast search program. The developmental expression patterns of novel genes in the cerebral cortex were investigated with in situ hybridization histochemistry on the developing and adult rat brain sections. Among the forty one developmentally differentially expressed cDNA bands, amplified with InEGA primer and TEAC primer by ODD PCR, twenty novel genes were screened by sequencing and sequence analysis with blast search program. Through the investigation of developmental expression pattern with in situ hybridization histochemistry, specific expression of five novel genes in the developing rat cerebral cortex was identified. 20-E14-2 was highly expressed in the cerebral cortex during the period of neurogenesis. The expression of 20-E20-1, 20-E20-6b, and 20-P0-5 was relatively well matched with neuronal cell migration in the cerebral cortex. And the strong expression of 20-P0-8b was observed in the neuronal cells of cerebral cortex during the period of syanptogenesis. From these results, it may be suspected that the five novel genes play roles in the development of cerebral cortex.

Use of Computer Telecommunication for Research and Education: Runnin ANATOMY Forum in Korean BBS for 1 year / 대한의료정보학회지

"The computer-based electronic academy of anatomy as the ideal goal", we made this concept to a reality by establishing ANATOMY forum in a nationwide bulletin board system. The concept of ANATOMY forum was designed at a small group meeting of members in Korean Association of Anatomist on October, 1992. Beginning on October 8, 1993, ANATOMY forum offers bulletins, file transfers and citizen band emulation for the anatomist as well as anyone who wants to join. We are providing library area for file transfer with four different library sections-EDUCATION, RESEARCH, INFORMATION OF ACADEMIC SOCIETY and ANATOMY IN GENERAL. In the bulletins we have two kinds of message area. One includes five message sections for the use of all members, consisting of NOTICE, LIBRARY INFORMATION, FOR MEDICAL PERSONNEL, QUESTION and ANSWER and MISCELLANEOUS NOTEBOOK. The another message area is for the executive use of anatomist, including five sections-EDUCATION, LABORATORY, RESTING PLACE, ACADEMIC SOCIETY and CONFERENCE ROOM. Currently we have 115 useful files in the library section, and more than 4,000 informative messages in the bulletins. The number of members of ANATOMY forum is now over than 1,000 including 48 anatomists of the most medical schools in Korea. The majority of the members are medical personnel and medical students. The ANATOMY forum is a group of people who wants to exchange ideas and informations on medical science and anatomy-related topics.